Laser-Stimulated Fluorescence in Paleontology

Laser-Stimulated Fluorescence in Paleontology

Fluorescence using ultraviolet (UV) light has seen increased use as a tool in paleontology over the last decade. Laser-stimulated fluorescence (LSF) is a next generation technique that is emerging as a way to fluoresce paleontological specimens that remain dark under typical UV. A laser's ability to concentrate very high flux rates both at the macroscopic and microscopic levels results in speci...

Laser-Induced Fluorescence Imaging

Fluorescence imaging is a tool of increasing importance in aerodynamics, fluid flow visualization, and nondestructive evaluation in a variety of industrial circumstances. It is a means for producing two-dimensional images of real surfaces or fluid case-sectional areas that correspond to data on properties such as temperature or pressure across the surfaces. This article discusses three major fl...

Supercontinuum stimulated emission depletion fluorescence lifetime imaging.

Supercontinuum (SC) stimulated emission depletion (STED) fluorescence lifetime imaging is demonstrated by using time-correlated single-photon counting (TCSPC) detection. The spatial resolution of the developed STED instrument was measured by imaging monodispersed 40-nm fluorescent beads and then determining their fwhm, and was 36 ± 9 and 40 ± 10 nm in the X and Y coordinates, respectively. The ...

Fluorescence imaging of electrically stimulated cells.

Designing high-throughput screens for voltage-gated ion channels has been a tremendous challenge for the pharmaceutical industry because channel activity is dependent on the transmembrane voltage gradient, a stimulus unlike ligand binding to G-protein-coupled receptors or ligand-gated ion channels. To achieve an acceptable throughput, assays to screen for voltage-gated ion channel modulators th...

Laser Excited Fluorescence of Dityrosine